[1]张安东,王 磊,陶榆玮,等.Pyrrosia petiolosa抗菌酯提物提取条件优化及效果[J].四川师范大学学报(自然科学版),2019,(05):697-702.[doi:10.3969/j.issn.1001-8395.2019.05.019]
 ZHANG Andong,WANG Lei,TAO Yuwei,et al.Extracting Condition Optimization of Pyrrosia petiolosa Ethylacetate Extract and Preliminary Antimicrobial Activity Investigation[J].Journal of SichuanNormal University,2019,(05):697-702.[doi:10.3969/j.issn.1001-8395.2019.05.019]
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Pyrrosia petiolosa抗菌酯提物提取条件优化及效果()
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《四川师范大学学报(自然科学版)》[ISSN:1001-8395/CN:51-1295/N]

卷:
期数:
2019年05期
页码:
697-702
栏目:
基础理论
出版日期:
2019-07-15

文章信息/Info

Title:
Extracting Condition Optimization of Pyrrosia petiolosa Ethylacetate Extract and Preliminary Antimicrobial Activity Investigation
文章编号:
1001-8395(2019)05-0697-06
作者:
张安东1 王 磊1 陶榆玮1 宋丽菊1 成 娟1 易思君1 吴道艳1 黄 敏2 赵 建1*
1.四川大学 生命科学学院, 四川 成都 610064; 2.四川省原子能研究院 辐照保藏四川省重点实验室, 四川 成都 610101
Author(s):
ZHANG Andong1 WANG Lei1 TAO Yuwei1 SONG Liju1 CHENG Juan1 YI Sijun1 WU Daoyan1 HUANG Min2 ZHAO Jian1
1.College of Life Science, Sichuan University, Chengdu 610064, Sichuan; 2.Irradiation Preservation Technology Key Laboratory of Sichuan Province, Sichuan Institute of Atomic Energy, Chengdu 610101, Sichuan
关键词:
石韦 金黄色葡萄球菌 正交试验 提取优化 拮抗
Keywords:
Pyrrosia petiolosa Staphylococcus aureus orthogonal test condition optimization antagonism
分类号:
Q94
DOI:
10.3969/j.issn.1001-8395.2019.05.019
文献标志码:
A
摘要:
优化Pyrrosia petiolosa酯提物提取条件,初步研究其拮抗Staphylococcus aureus作用效果.采用正交试验优化提取条件,牛津杯法测定Pyrrosia petiolosa酯提物拮抗S.aureus活性; 倍比稀释法测定其拮抗S.aureus的MIC和MBC值; 扫描电镜观察PPEAE处理S.aureus后形态变化.正交试验结果显示:质量浓度1/20 g/mL、提取温度60 ℃、浸提时间5 d为最优提取条件,优化后酯提物抑菌效率可提高25.7%.Pyrrosia petiolosa
Abstract:
In this paper, we optimize extracting condition of Pyrrosia petiolosa ethylacetate extract and investigate its antimicrobial effect on Staphylococcus aureus.Orthogonal test, Oxford cup, dilution method and SEM were employed to optimize extracting condition, measure the size of inhibition zone, determine MIC and MBC value and observe the changes in cell morphology.The optimal condition was extracting temperature at 60 ℃, soaking time at 5 d and material to solvent ratio at 1/20 g/mL and inhibition efficiency against S.aureus increases by 25.7% after optimization.Inhibition zone diameter was 8 and 24 mm when ethylacetate extract concentration was 1 and 50 mg/mL, respectively.Its MIC and MBC value were 7.8 and 15.6 mg/mL.SEM scan results showed that S.aureus partial cell surface collapse and shrink after exposed to Pyrrosia petiolosa ethylacetate extract.Inhibition efficiency of Pyrrosia petiolosa ethylacetate extract against S.aureus increases by 25.7% after optimization and it's a potent antagonist for S.aureus.

参考文献/References:

[1] YANG Y C, YANG C, MO S Y, et al.A new flavonol diglycoside from Pyrrosia petiolosa[J].Chin Cheml Lett,2003,14(9):920-922.
[2] 战妍妃,李媛媛,安欣欣,等.石韦的抗病毒黄酮部位及有效化学成分[J].暨南大学学报(自然科学与医学版),2018,39(6):470-480.
[3] 徐红,范琳琳,欧丽悦,等.黔产庐山石韦绿原酸及芒果苷含量的快速检测方法[J].贵州农业科学,2018,46(10):134-136.
[4] SONG L J, CAO M, CHEN C, et al.Antibacterial activity of Pyrrosia petiolosa ethyl acetate extract against Staphylococcus aureus by decreasing hla and sea virulence genes[J].Natural Product Research,2017,31(11):1347-1350.
[5] 杨凤华,康成,李淑华,等.黄芪水溶性黄酮类对小鼠细胞免疫功能的影响[J].时珍国医国药,2002,13(12):718-719.
[6] 张正海,董艳,姬妍茹,等.汉麻叶提取物抑菌活性及抑菌稳定性研究[J].食品安全质量检测学报,2019,10(4):927-933.
[7] WANG N, WANG J H, LI X, et al.Flavonoids from Pyrrosia petiolosa (Christ)Ching[J].J Asian Natural Products Research,2006,8(8):753-756.
[8] YANG C, SHI J G, MO S Y, et al.Chemical constituents of Pyrrosia petiolosa[J].J Asian Natural Products Research,2003,5(2):143-150.
[9] SHIBABAW A, ABEBE T, MIHRET A.Antimicrobial susceptibility pattern of nasal Staphylococcus aureus among Dessie Referral Hospital health care workers, Dessie, Northeast Ethiopia[J].International J Infectious Diseases,2014,25(14):22-25.
[10] 马金柱,张少铎,程欣然,等.金黄色葡萄球菌CCT融合蛋白的表达及免疫活性研究[J].黑龙江八一农垦大学学报,2017,29(6):40-44.
[11] 杨涵,刘庆中.金黄色葡萄球菌杀白细胞素ED的研究进展[J].微生物与感染,2017,12(6):385-390.
[12] STINGLEY R L, LIU H, MULLIS L B, et al.Staphylococcus aureus toxic shock syndrome toxin-1(TSST-1)production and Lactobacillus species growth in a defined medium simulating vaginal secretions[J].J Microbiological Methods,2014,106(11):57-66.
[13] ANSTEAD G M, CADENA J, JAVERI H.Treatment of infections due to resistant Staphylococcus aureus[J].Methods in Molecular Biology,2014,1085(1):259-309.
[14] SPAULDING A R, LIN Y C, MERRIMAN J A, et al.Immunity to Staphylococcus aureus secreted proteins protects rabbits from serious illnesses[J].Vaccine,2012,30(34):5099-5109.
[15] LV F, LIANG H, YUAN Q, et al.In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms[J].Food Research International,2011,44(9):3057-3064.
[16] BASANISI M G, LA B G, NOBILI G, et al.Genotyping of methicillin-resistant Staphylococcus aureus(MRSA)isolated from milk and dairy products in South Italy[J].Food Microbiology,2016,62(10):141-146.
[17] WANG S Y, SUN Z L, LIU T, et al.Flavonoids from Sophora moorcroftiana and their synergistic antibacterial effects on MRSA[J].Phytotherapy Research Ptr,2014,28(7):1071-1076.
[18] LIAU B C, PONNUSAMY V K, LEE M R, et al.Development of pressurized hot water extraction for five flavonoid glycosides from defatted Camellia oleifera seeds(byproducts)[J].Industrial Crops & Products,2017,95(1):296-304.
[19] GE Y, MA R K, WANG L S, et al.Optimization of the water extraction technology of flavonoids in Thlaspi arvense linn[J].Advanced Materials Research,2013,781(9):1194-1198.
[20] TUKUR A, BELLO I A, KOORBANALLY N A, et al.Syntheses and antibiotic evaluation of 2-{[(2R,4R)-4-carboxy-2-hydroxypyrrolidin-1-yl] carbonyl} benzene-1,5-dicarboxylic acids and 2-carbamoylbenzene-1,5-dicarboxylic acid analogues[J].International J Medicinal Chemistry,2016,2016(2):1-8.
[21] 刘刚,伍佩珂,蒋小妹,等.桂花果皮醇提物的抑菌活性和抗氧化活性的研究[J].四川师范大学学报(自然科学版),2018,41(5):672-676.
[22] 汪超,唐明,魏琴.连香树精油对植物病原真菌抑菌作用及对人肝癌细胞株SMMC-7721细胞毒性研究[J].四川师范大学学报(自然科学版),2016,39(5):735-742.
[23] 周本宏,松长青,姜姗,等.地榆鞣质提取物的抗菌活性及对金黄色葡萄球菌的抑菌机制研究[J].中国药师,2016,19(3):464-469.
[24] STODDART A,H ERTZ M I, DAVID M, et al.Determination of antibacterial mode of action of allium sativum essential oil against foodborne pathogens using membrane permeability and surface characteristic parameters [J].J Food Safety,2013,33(2):197-208.
[25] ZHANG Y, LIU X, WANG Y, et al.Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus[J].Food Control,2016,59(1):282-289.

备注/Memo

备注/Memo:
收稿日期:2018-05-17 接受时期:2018-07-04
基金项目:四川省重点研发项目(2017FZ0059)和四川省原子能研究院辐照保藏四川省重点实验室开放基金(FZBC2018003)
*通信作者简介:赵 建(1976—),男,教授,主要从事资源微生物及抗病原微生物天然免疫的研究,E-mail:zj804@163.com
更新日期/Last Update: 2019-07-15