[1]许崇利,许崇波,林一民*.大肠杆菌K88ac-ST1-LTB融合蛋白多价卵黄抗体的制备[J].四川师范大学学报(自然科学版),2020,43(03):378-382.[doi:10.3969/j.issn.1001-8395.2020.03.014]
 XU Chongli,XU Chongbo,LIN Yimin.Preparation of Multivalent Egg Yolk Antibody of Enterotoxigenic Escherichia coliFusion Protein K88ac-ST1-LTB[J].Journal of SichuanNormal University,2020,43(03):378-382.[doi:10.3969/j.issn.1001-8395.2020.03.014]
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大肠杆菌K88ac-ST1-LTB融合蛋白多价卵黄抗体的制备()
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《四川师范大学学报(自然科学版)》[ISSN:1001-8395/CN:51-1295/N]

卷:
43卷
期数:
2020年03期
页码:
378-382
栏目:
基础理论
出版日期:
2020-05-05

文章信息/Info

Title:
Preparation of Multivalent Egg Yolk Antibody of Enterotoxigenic Escherichia coliFusion Protein K88ac-ST1-LTB
文章编号:
1001-8395(2020)03-0378-05
作者:
许崇利12 许崇波3 林一民4*
1. 吉林化工学院 生物与食品工程学院, 吉林 吉林 132022; 2. 重庆医药高等专科学校 医学技术学院, 重庆 401331; 3. 韶关学院 英东生命科学院, 广东 韶关 512005; 4. 重庆大学附属肿瘤医院 肿瘤转移与个体化诊治转化研究重庆市重点实验室, 重庆 400030
Author(s):
XU Chongli12 XU Chongbo3 LIN Yimin4
1. College of Biology and Food Engineering, Jilin Institute of Chemical Technology, Jilin 132022, Jilin; 2. College of Medical Technology, Chongqing Medical and Pharmaceutical College, Chongqing 401331; 3. Yingdong College of Life Sciences, Shaoguan University, Shaoguan 512005, Guangdong; 4. Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing 400030
关键词:
大肠杆菌 K88ac-ST1-LTB基因 重组表达 卵黄抗体
Keywords:
Escherichia coli K88ac-ST1-LTB gene recombinant expression egg yolk antibodies
分类号:
S852; Q78
DOI:
10.3969/j.issn.1001-8395.2020.03.014
文献标志码:
A
摘要:
对已构建的重组菌株BL21(DE3)(pETXKSL3)进行鉴定,酶切鉴定证实重组质粒pETXKSL3含有K88ac-ST1-LTB融合基因且阅读框架正确.Western blot分析表明K88ac-ST1-LTB融合蛋白能够被ST1单抗、K88ac和LTB抗体识别.以K88ac-ST1-LTB融合蛋白为抗原免疫蛋鸡制备卵黄抗体,E
Abstract:
By identifying the BL21(DE3)(pETXKSL3)recombinant strain was identified and restriction endonuclease digestion confirmed that the pETXKSL3 recombinant plasmid contained the K88ac-ST1-LTB fusion gene and the reading frame was correct. Western blot analysis showed that the K88ac-ST1-LTB fusion protein was recognized by ST1 mAb, K88ac and LTB antibodies. Egg yolk antibody was prepared by immunizing hens with K88ac-ST1-LTB fusion protein as antigen. The titer of yolk antibody can reach 1:13 200 by ELISA. Piglet challenge experiments showed that the effective rate of the prepared egg yolk antibody was 96% against yellow scours of newborn piglets and white scours of piglets. The above results indicate that K88ac-ST1-LTB multivalent yolk antibody has a good preventive effect on newborn piglet diarrhea caused by ETEC. Therefore, it has laid a solid foundation for the development of a high-efficiency alternative antibiotic yolk antibody which can prevent the newborn piglet diarrhea caused by ETEC.

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备注/Memo

备注/Memo:
收稿日期:2019-11-06 接受日期:2019-11-28 基金项目:吉林省教育厅“十三五”科学技术研究项目(JJKH20190838KJ) *通信作者简介:林一民(1965-),女,副教授,主要从事临床血液和体液检验研究,E-mail:1966128966@qq.com
更新日期/Last Update: 2020-05-05