[1]艾立瑶,秦东旭,王力波,等.香芹酚对H2O2诱导神经细胞突触损伤的修复作用[J].四川师范大学学报(自然科学版),2020,43(04):550-554.[doi:10.3969/j.issn.1001-8395.2020.04.020]
 AI Liyao,QIN Dongxu,WANG Libo,et al.Repair Effect of Carvacrol on Synaptic Damage Induced by H2O2 in Neurons[J].Journal of SichuanNormal University,2020,43(04):550-554.[doi:10.3969/j.issn.1001-8395.2020.04.020]
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香芹酚对H2O2诱导神经细胞突触损伤的修复作用()
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《四川师范大学学报(自然科学版)》[ISSN:1001-8395/CN:51-1295/N]

卷:
43卷
期数:
2020年04期
页码:
550-554
栏目:
基础理论
出版日期:
2020-06-20

文章信息/Info

Title:
Repair Effect of Carvacrol on Synaptic Damage Induced by H2O2 in Neurons
文章编号:
1001-8395(2020)04-0550-05
作者:
艾立瑶 秦东旭 王力波 宋 英*
浙江工业大学 药学院, 浙江 杭州 310014
Author(s):
AI Liyao QIN Dongxu WANG Libo SONG Ying
College of Pharmacy, Zhejiang University of Technology, Hangzhou 310014, Zhejiang
关键词:
PC12 过氧化氢 神经元细胞 突起 凋亡
Keywords:
PC12 hydrogen peroxide neuronal cells protrusion apoptosis
分类号:
R964
DOI:
10.3969/j.issn.1001-8395.2020.04.020
文献标志码:
A
摘要:
主要探究香芹酚在神经细胞中对于神经突起生长的影响.首先,应用不同浓度H2O2处理PC12细胞建立神经细胞损伤模型; 其次,将药物CA作用于处理后的PC12细胞,用MTT检测细胞活力、Hoechst33342染色观察细胞凋亡的变化; 最后,在光镜下观察CA对于细胞突起的影响.另外,培养神经元细胞用神经生长因子作为阳性对照,进一步研究CA对神经细胞突起生长的影响,发现:浓度400 μmol/L H2O2能明显地诱导PC12细胞凋亡,增加细胞凋亡率,导致神经突起短缩; 而浓度0.01 mmol/L的CA能明显降低细胞凋亡率,使神经突起延伸,对神经元细胞突起生长也有一定的促进作用.研究结果显示CA可保护神经
Abstract:
It mainly explores the effect of carvacrol on the growth of neurites in nerve cells. Firstly, different concentrations of H2O2 were used to treat PC12 to establish a neuronal injury model. The drug CA was further applied to the treated PC12. Cell viability was detected by MTT and Hoechst33342 staining was used to observe the changes of apoptosis. The effect of CA on cell processes was observed under light microscope. In addition, neuronal cells were cultured with nerve growth factor as a positive control to further investigate the effect of CA on the growth of nerve cell processes. The results showed that 400 μmol/L H2O2 could significantly induce apoptosis of PC12 cells, increase apoptosis rate, and lead to neurite outgrowth. The 0.01 mmol/L CA can significantly reduce the apoptotic rate, extend the neurites, and promote the growth of neuronal cells. The results show that CA can protect nerve cell damage, reduce neuronal apoptosis and promote neurite outgrowth. This study provides a theoretical basis for the treatment of nerve injury.

参考文献/References:

[1] HAMAMCIOGLU B, KOCANCI F G, ASLIM B. Phytochemical screening and evaluation of neuroprotective, anti-mutagenic and anti-genotoxic effects of Turkish endemic Glaucium acutidentatum[J]. S Afr J Bot,2018,117:232-239.
[2] SUNTRES Z E, COCCIMIGLIO J, ALIPOUR M. The bioactivity and toxicological actions of carvacrol[J]. Crit Rev Food Sci Nutr,2015,55(3):304-318.
[3] KISKO G, ROLLER S. Carvacrol and p-cymene inactivate Escherichia coli O157:H7 in apple juice[J]. BMC Microbiol,2005,5:36.
[4] LINS M F, SOUZA J M M, BISPO A M, et al. Carvacrol prevents impairments in motor and neurochemical parameters in a model of progressive parkinsonism induced by reserpine[J]. Brain Res Bull,2018,139:9-15.
[5] ZOTT M, COLAIANNA M, MORGESE P, et al. Carvacrol:from ancient flavoring to neuromodulatory agent[J]. Molecules,2013,18(6):6161-6172.
[6] DENG W, LU H, TENG J. Carvacrol attenuates diabetes-associated cognitive deficits in rats[J]. J Mol Neurosci,2013,51(3):813.
[7] LI G, TIAN Y, ZHANG Y, et al. A novel ligustrazine derivative T-VA prevents neurotoxicity in differentiated PC12 cells and protects the brain against ischemia injury in MCAO rats[J]. Inter J Mol Sci,2015,16(9):21759-21774.
[8] MO S J, HONG J, CHEN X, et al. VEGF-mediated NF-κB activation protects PC12 cells from damage induced by hypoxia[J]. Neuroscience Letters,2016,610:54-59.
[9] HAAS A J, LE P Y, ZHADOBOV M, et al. Effects of 60-GHz millimeter waves on neurite outgrowth in PC12 cells using high-content screening[J]. Neuroscience Letters,2016,618:58-65.
[10] TIAN X, YUE R, ZENG H, et al. Distinctive effect on nerve growth factor-induced PC12 cell neurite outgrowth by two unique neolignan enantiomers from Illicium merrillianum[J]. Scientific Reports,2015,5:16982.
[11] GUO Z, PAN R Y, QIN X Y. Potential protection of Coeloglossum viride var.bracteatum extract against oxidative stress in rat cortical neurons[J]. J Anal Methods Chem,2013,5:326570.
[12] PASTORIN G, MARCHESAN S, HOEBEKE J, et al. Design and activity of cationic fullerene derivatives as inhibitors of acetylcholinesterase[J]. Org Biomol Chem,2006,4:2556-2562.
[13] MAURO A G, MEZZAROMA E, TORRADO J, et al. Reduction of myocardial ischemia-reperfusion injury by inhibiting interleukin-1 alpha[J]. Cardiovasc Pharmacol,2017,69(3):156-160.
[14] NEVES G, COOKE S F, BLISS T V. Synapticptic plasticity, memory and the hippocampus:a neural network appriach to causality[J]. Nat Rev Neurosci,2008,9(1):65-75.

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备注/Memo

备注/Memo:
收稿日期:2019-06-06 接受日期:2019-08-22
基金项目:浙江省自然科学基金(LY17H090018)
*通信作者简介:宋 英(1968—),女,副教授,主要从事细胞生物学和神经药理学的研究,E-mail:songying@zjut.edu.cn
更新日期/Last Update: 2020-06-20